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Fig. 6. Crustacean hyperglycemic hormone (CHH)-like, CHH precursor-related peptide
(CPRP)-like and mandibular organ-inhibiting hormone (MOIH)-like labeling in
the segmental nerves (sn) and pericardial organ (PO) of Cancer
productus. (A) CHH-like labeling in the sn anterior to the PO.
Two bipolar somata were labeled with the CHH antibody in the anterior bar of
the PO or in the sns just anterior to it (one stained soma shown;
arrow). Staining in these somata was uniform in its distribution, with the
label filling the axons emanating from the cell bodies. As this micrograph
shows, many small diameter axons and superficially located nerve terminals
were also labeled by the CHH antibody in the sn. This micrograph is a
brightest pixel projection of 34 optical sections taken at 1.95 µm
intervals. (B) CPRP-like labeling in the sn anterior to the PO. As
with the CHH antibody, two bipolar somata were labeled in the
sn/anterior bar area of the PO by the CPRP antibody (two shown;
arrows). Here, labeling within the somata was granular in appearance, and
little immunoreactivity was present in the axons emanating from the cell
bodies. Similar to the CHH label, many small diameter axons and superficially
located nerve terminals in the sn were stained by the CPRP antibody.
This micrograph is a brightest pixel projection of 21 optical sections taken
at 1.95 µm intervals. (C) CHH-like labeling in the anterior bar of the PO.
In addition to the bipolar neurons seen in the sn, a single
multipolar soma was routinely labeled by the CHH antibody in the anterior bar
of the PO (arrow). As with the other PO somata labeled by this antiserum,
staining in this cell body was uniform and diffuse in appearance. In addition
to the soma, an extensive network of fine fibers, as well as superficial nerve
terminals, were labeled by the CHH antibody within the anterior bar of the PO.
Although not shown, the CPRP antibody produced an identical pattern of
labeling in this portion of the PO, with the exception that labeling in the
multipolar soma was granular in appearance rather than uniformly diffuse. This
micrograph is a brightest pixel projection of 37 optical sections taken at
1.95 µm intervals. (D) MOIH-like labeling in the anterior bar of the PO. As
with the CHH and CPRP labels, the MOIH antibody stained a network of fine
fibers and superficially located nerve terminals in the anterior bar of the
PO, though the immunoreactivity was less extensive and weaker than with the
other sera. However, no somata were labeled by the MOIH antiserum, nor were
any labeled in the sns near the anterior bar. This micrograph is a
brightest pixel projection of 26 optical sections taken at 1.95 µm
intervals. All scale bars, 100 µm.
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