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Fig. 5. Double-immunolabeling of the X-organ (XO) with antisera to crustacean
hyperglycemic hormone (CHH)-family members and CHH precursor-related peptide
(CPRP) in Cancer productus. (A1-3) Moult-inhibiting hormone
(MIH)/mandibular organ-inhibiting hormone (MOIH) double labeling in the XO.
This series of micrographs consists of pseudo-colored single optical sections
collected from a single focal plane in an XO labeled with both anti-MIH (A1,
pseudo-colored green) and anti-MOIH (A2, pseudo-colored red). When A1 and A2
were merged (A3), complete overlap in the labeled structures was revealed
(i.e. yellow, but not green or red, coloration is seen in all immunopositive
structures in the micrograph), suggesting that the MIH- and MOIH-like labels
are contained in a common set of XO somata. The same result was seen in
preparations pairing anti-CHH/anti-CPRP (data not shown). (B1-3) MIH/CPRP
double labeling in the XO. This series of micrographs consists of
pseudo-colored single optical sections collected from a single focal plane in
an XO labeled with both anti-MIH (B1, pseudo-colored green) and anti-CPRP (B2,
pseudo-colored red). When B1 and B2 were merged (B3), no overlap in the
labeled structures was revealed (i.e. a lack of yellow colored structures in
the micrograph), suggesting that the MIH- and CPRP-like labels are contained
in distinct sets of XO somata. Though not shown, the same result was seen in
preparations pairing anti-MIH/anti-CHH, anti-MOIH/anti-CHH and
anti-MOIH/anti-CPRP. A1,2 and B1,2 are all shown at the same scale. Likewise
A3 and B3 are shown at the same magnification. Scale bars in B2 and B3, 100
µm.
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