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Figure 3


Fig. 3. Moult-inhibiting hormone (MIH)-like immunoreactivity in the X-organ-sinus gland (XO-SG) system of Cancer productus. (A) MIH-like labeling in the SG. Whole-mount immunolabeling of the nervous system contained within the eyestalk with antibody to MIH consistently stained nerve terminals in the SG. This labeling could be unambiguously traced via immunopositive axons in the sinus gland tract (sgt) to somata in the XO. This micrograph is a brightest pixel projection of 29 optical sections taken at 1.95 µm intervals. (B) MIH-like labeling in the XO. Immunoprocessing using anti-MIH consistently labeled 30 or so somata in the XO. This micrograph is a brightest pixel projection of 19 optical sections taken at 1.95 µm intervals. A and B are taken from the same preparation and are shown at the same magnification. Scale bar in B, 150 µm.





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