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Figure 1


Fig. 1. Distribution of major inorganic components in a Nereis jaw cross section. (A) Scanning electron microscopy (SEM) image of a complete Nereis jaw. Embedded jaws were prepared by trimming to the plane indicated (red disc) with a diamond knife. Energy dispersive X-ray spectroscopy (EDS) maps of (B) untreated, (C) Tris-treated and (D) EDTA-treated jaw surfaces. Untreated and Tris-treated images are of a single jaw (EDTA treatment was done on a different jaw - distributions of Zn, Cl and Br prior to EDTA treatment were indistinguishable to those in untreated jaws). EDTA treatment, but not incubation in Tris alone, removes Zn and Cl in three distinct regions of the jaw cross section (white arrows). Zn loss is more prevalent in Br-free regions of the sample. Scale bar, 100 µm (cross sections only).





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