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Fig. 9. Spatial expression of agNAT8 transcript in the alimentary canal, CNS and
PNS of An. gambiae larvae. (A) In situ hybridization of
agNAT8-specific probes in the whole-mount midgut from 4th instar An.
gambiae larvae. (B) Magnified image of the rectal gland. (C) Intensive
hybridization in the salivary gland. (D) Stretched-opened esophageal
epithelial infolding, cardia; this epithelial region is associated with
secretion of the tubular peritrophic membrane. (E) Area-specific labeling in
the isolated neuronal ventral cord of CNS; three thoracic ganglia are shown.
(F,G) Dorsal and lateral views of agNAT8 hybridization pattern in the larval
stemma (eye); this labeling is associated with primary photosensitive neurons
(green arrows). (H) An example of hybridization of sensory neurons associated
with vibration-sensitive hair sensilla on the larval head (black arrow). (I)
Labeling in the basal part of the peg sensillum at the head capsule (white
arrow); this structure has an unknown sensory role in larvae, but it is
morphologically similar to temperature-sensitive structures in adults. (J)
agNAT8-pecific hybridization of specific populations of chemosensory neurons
in maxillary pulp (MP) and labium afferents (LB) (open arrows). AMG, anterior
midgut; CA, cardia, a sub-esophageal invagination; GC, gastric caeca; MT,
Malpighian tubules; PMG, posterior midgut. All images were acquired using a
Hoffman contrast microscope except for A and E, which were acquired using a
stereo microscope. Bars, 1000 µm (A), 200 µm (B-E), 100 µm (F-H,J),
50 µm (I).
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