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Figure 6


Fig. 6. Electrical properties of agNAT8 transporter. (A) Families of current traces induced by square pulse polarization of oocyte membranes in a 98 mmol l-1 NaCl solution (left) and after application of 2 mmol l-1 phenylalanine (right) (Vhold, holding voltage=-50 mV, first pulse=+30 mV, last pulse=-120 mV; increment=-10 mV). (B) Family of I/V plots upon ion substitution in the bathing media. Line colors and thickness represent different ion and substrate profiles, respectively (see inserts). (C) I/V profiles with subtracted `control' leak currents in the absence of substrate molecules. Phe concentration in B and C = 1 mmol l-1.





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