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Fig. 6. Electrical properties of agNAT8 transporter. (A) Families of current traces
induced by square pulse polarization of oocyte membranes in a 98 mmol
l-1 NaCl solution (left) and after application of 2 mmol
l-1 phenylalanine (right) (Vhold, holding
voltage=-50 mV, first pulse=+30 mV, last pulse=-120 mV; increment=-10 mV). (B)
Family of I/V plots upon ion substitution in the bathing
media. Line colors and thickness represent different ion and substrate
profiles, respectively (see inserts). (C) I/V profiles with
subtracted `control' leak currents in the absence of substrate molecules. Phe
concentration in B and C = 1 mmol l-1.