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Figure 10


Fig. 10. Quantitative real-time PCR analysis of agNAT8 expression in distinct larval tissues and developmental stages of An. gambiae. (A) Relative expression levels of agNAT8 in tissues of 4th instar larvae. (B) Relative expression levels of agNAT8 at various developmental stages and in adult tissues. Note the different scales on the x axis for A and B. Data are represented as the mean of three averaged replicates of two independent experiments plus standard error. An. gambiae 18S ribosomal RNA was used as a reference gene. Results were normalized to values for whole larvae, which was set to a value of 1.0. CNS, central nervous system; SG, salivary glands; GC, gastric caeca; AM, anterior midgut; PM, posterior midgut; MT, Malpighian tubules; RG, rectal gland; rep org, reproductive organs.





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