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Fig. 4. Changes in pHi upon challenging cells with hypertonicity while using (a)
Ca2+-containing medium (control), (b) Ca2+-free medium
and (c) Ca2+-free medium along with preincubation of cells with the
intracellular Ca2+-chelating agent BAPTA-AM. Values are means
± s.e.m. of 68-95 cells from 5-10 independent preparations. Inset: pHi
changes upon exposing cells to 25 µmol l-1 BAPTA-AM followed by
Ca2+-free medium. Values are means ± s.e.m. of 46 cells from
three independent preparations.