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Fig. 5. Phylogeny of lepidopteran blue opsin genes. The tree is based upon a
neighbor-joining (NJ) analysis of 1077 nucleotide sites, using Tamura-Nei
distance and heterogeneous pattern of nucleotide substitution among lineages.
Bootstrap values shown are based upon 500 maximum likelihood (ML) bootstrap
replicates determined using the GTR+
+I model with estimated gamma shape
parameter=0.574 and proportion of invariant sites=0.1474. GenBank accession
numbers for sequences are provided in supplementary material Table S3. Inset:
Dorsal wing of a male Polyommatus icarus, one of the lycaenids
surveyed.