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Fig. 1. Specific amino acids (AAs) are essential for 20-hydroxyecdysone
(20E)-activation of the vitellogenin (vg) gene. Fat bodies
from 3- to 5-day-old mosquitoes were cultured for 3 h at 27°C in media
lacking individual AAs in the presence of 20E (10-6 mol
l-1). Total RNA was isolated from three groups of six fat bodies
per treatment. cDNA was synthesized from equal amounts of DNase I-treated
total RNA. Real-time PCR was used to quantify levels of vg mRNA. Data
was normalized by real-time PCR analysis of actin levels in the cDNA
samples. vg production in responses to 20E stimulation in the
`withdrawal' medias are presented as the mean percentage (± s.e.m. of
triplicate samples) relative to the response observed in the control medium
containing complete AAs.
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