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Fig. 5. In vitro haemoglobin-oxygen binding curves for cod blood incubated
at the fish's acclimation temperature (7°C), or after incubation
temperature was increased to 20 or 24°C. Haematocrit was initially set at
20%, and changes in PO2 were made every 30 min. The lines
were fitted to the data for each temperature using a 4-parameter sigmoidal
function. Six individuals were used to generate each curve. See
Table 2 for statistical
analyses of parameters that define Hb-O2 affinity and binding
capacity.
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