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Fig. 1. Heat stress induces fluorescence of the NO-sensitive probe DAF-FM in A.
pallida. (A) Optical cross-section through a tentacle of symbiotic A.
pallida incubated at an ambient temperature of 25°C (left) and after
24 h of heat stress at 33°C (right), with inset showing expelled host
cells with algae. The DAF-FM (510-530 nm) NOdependent fluorescent signal is
colored yellow; algal autofluorescence is colored red. (B) Quantification of
relative fluorescence intensity of NO-sensitive DAF-FM (510-530 nm) in
tentacles as a function of heat stress (N=3 anemones per treatment;
bars represent means + s.d.; t-test, P=0.0015).
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