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Fig. 2. Depolarization in high external K+ causes CBF excitation. (A) 30
mmol l-1 K+ bath (filled circles) significantly
increases CBF as compared to ASW controls (N=5, P
0.0003)
in CPE cell explants. After 3 min, 30 mmol l-1 K+ is
still excitatory (P
0.02) but less so. After 6 min, 30 mmol
l-1 K+ is no longer excitatory. 30 mmol l-1
K+ seawater was not excitatory in a zero Ca2+ bath (open
squares, N=6), and only transiently so (P
0.02) in a bath
containing 50 µmol l-1 of the Ca2+ channel blocker
nifedipine (triangles, N=5). (B) 30 mmol l-1 K+
significantly excites CBF in isolated, individual CPE cells (N=5,
P
0.007). *Significant change from mean basal
levels.