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Figure 3


Fig. 3. Large portion of whole cell current is carried by Cl- ions. (A) Representative voltage clamp records of currents (elicited from seven +20 mV step increases, beginning from -60 mV) from a cell in low (132 mmol l-1) Cl- bath solution (shadow, a representative trace from a different cell in ASW at +63 mV). Open arrowhead, position of fast I-V; filled arrowhead, position of slow I-V. (B) Slow I-V reveals the cells in the low Cl- bath (triangles, N=18) have substantially reduced currents as compared to those in normal ASW (circles, N=76). (B,inset) Comparison of maximum current sizes between ASW (73 mV; N=76) and Low Cl- (70 mV; N=18) reveal a significantly smaller current amplitude for those cells in a Low Cl- bath (P=2.4x10-11). (C) Fast I-V shows an even larger difference in current amplitude (circles, ASW; N=76; triangles, Low Cl-; N=18). (C,inset) Comparison of current amplitudes after 40 ms of depolarization show a larger discrepancy in current amplitude between ASW at 73 mV and Low Cl- at 70 mV (P=3.4x10-11), suggesting that the Cl- current is the faster activating current. (D) Whole cell current amplitude is significantly reduced after the application of Cl- channel blockers DIDS and 9-AC (500 µmol l-1 DIDS P=0.01, N=4; 50 µmol l-1 9-AC P=0.01, N=4).





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