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Figure 2


Fig. 2. In situ patch method allows for successful recordings from ciliated epithelial cells. (Ai) Representative micrograph taken while recording using the in situ patch technique. Identifying whether the cell being recorded from is ciliated is not possible. Scale bar, 25 µm. (Aii) Representative voltage clamp records of currents elicited from eight +20 mV step increases beginning from -57 mV. (Bi) Representative micrograph taken while recording from a disassociated epithelia cell that can be clearly identified as ciliated. Scale bar, 25 µm. (Bii) Voltage clamp records from cell pictured in Bi of currents elicited from eight +20 mV step increases beginning from -57 mV. (C) Slow current/voltage relationship reveals no difference in kinetics or amplitude of currents recorded using the `blind' patch technique (circles, N=76) or from disassociated clearly identified ciliated cells (triangles, N=12).





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