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Fig. 2. In situ patch method allows for successful recordings from
ciliated epithelial cells. (Ai) Representative micrograph taken while
recording using the in situ patch technique. Identifying whether the
cell being recorded from is ciliated is not possible. Scale bar, 25 µm.
(Aii) Representative voltage clamp records of currents elicited from eight +20
mV step increases beginning from -57 mV. (Bi) Representative micrograph taken
while recording from a disassociated epithelia cell that can be clearly
identified as ciliated. Scale bar, 25 µm. (Bii) Voltage clamp records from
cell pictured in Bi of currents elicited from eight +20 mV step increases
beginning from -57 mV. (C) Slow current/voltage relationship reveals no
difference in kinetics or amplitude of currents recorded using the `blind'
patch technique (circles, N=76) or from disassociated clearly
identified ciliated cells (triangles, N=12).