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Fig. 5. Hsp40 mRNA (filled symbols) and protein (open symbols) in the (A) gill and
(B) white muscle from Gillichthys mirabilis. Average protein
concentrations and mRNA levels are shown for fish (N=4) from each
time point, either during heat shock (solid lines; circles) or during recovery
(broken lines; squares). At a given time point, fish were sacrificed and
frozen immediately in liquid nitrogen. The mRNA values are taken from the
microarray analyses (see Materials and methods and Figs
2,
3). For protein concentration
determination, thawed tissues were homogenized and dot-blotted onto
nitrocellulose membranes. Following incubation in primary and secondary
antibodies, blots were visualized with enhanced chemiluminescence reagent and
exposed to X-ray film; exposed film was scanned and spot intensity quantified
densitometrically. Average pixel intensity for each spot was normalized to the
average for the four time 0 individuals. For protein levels, values are means
± s.d. (N=4). *Heat shock time points that differ
significantly (ANOVA, P 
0.001) from time 0;
recovery time points that differ significantly from time 0.
In some cases, error bars are contained within symbols.
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