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Fig. 4. Ubiquitin-conjugated proteins in gill and white muscle from Gillichthys
mirabilis. For determination of the concentration of ubiquitin-conjugated
proteins, thawed tissues were homogenized and dot-blotted onto nitrocellulose
membranes. Following incubation in primary and secondary antibodies, blots
were visualized with enhanced chemiluminescence reagent and exposed to X-ray
film; exposed film was scanned and spot intensity quantified
densitometrically. Average pixel intensity for each spot was normalized to the
average for the four time 0 individuals. Values are means ± s.d.
(N=4). *Heat shock time points, in gill tissue, that
differ significantly (ANOVA, P 
0.001) from time 0;
recovery time points, in gill tissue, that differ
significantly from time 0; 
heat shock time points, in
muscle tissue, that differ significantly (P 0.001) from time 0.
In some cases, error bars are contained within symbols.
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