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Fig. 7. Substrate specificity of recombinant CuquEndo-containing supernatant on
different single- or double-stranded polynucleotides. Hydrolysis products were
resolved using an IEC-DEAE-NPR column. Recombinant CuquEndo hydrolyzed only
double-stranded DNA with no sequence specificity. No endonuclease activity was
detected on single-stranded substrates.