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Figure 5


Fig. 5. Effect of divalent cations on recombinant CuquEndo activity. Reaction mixtures containing 1 µl of recombinant CuquEndo and 500 ng of plasmid DNA in a final volume of 20 µl were incubated for 10 min at 37°C in buffers with cation concentration ranging from 0 to 10 mmol l-1. The digested DNA plasmids were resolved in 1.2% agarose gels and visualized under UV light. (A) MgCl2. (B) CaCl2.





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