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Figure 3


Fig. 3. Secretion of CuquEndo in Cx. quinquefasciatus saliva. Starved mosquitoes were allowed to probe in an agarose gel containing 10 mmol l-1 NaHCO3, 100 mmol l-1 NaCl, 1 mmol l-1 MgCl2, 50 mmol l-1 Tris, pH 7.5, and 200 µg ml-1 of double-stranded plasmid DNA. After 30 min incubation at 37°C, the gel was stained with Ethidium Bromide and visualized under UV light. Arrows indicate the hydrolysis of plasmid DNA at the biting sites within the gel.





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