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Figure 2


Fig. 2. Endonuclease activity in salivary gland extract of female Culex quinquefasciatus. Double-stranded plasmid DNA (400 ng) was incubated in a final volume of 20 µl with different salivary gland dilutions (serial dilutions of one salivary gland) and incubated for 10 min at 37°C. (A) 10 µl from each sample was electrophoresed in a 1.2% agarose gel and visualized under UV light. NSG, no salivary gland, negative control. (B) 5 µl from each reaction were diluted in 100 µl of Hoechst dye mix and the fluorescence measured using a fluorimeter with 365/460-nm (excitation/emission, 6.1 V) filters.





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