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Figure 5


Fig. 5. Effects of buffer composition on the response of efferent branchial arteries to addition of 40 mmol l–1 NH4Cl (intracellular alkalosis) and NH4Cl washout (Wash; intracellular acidosis) 30 min later. Addition of 10 mmol l–1 NaHCO3 to Hepes (Hepes +HCO3–) did not affect the contraction accompanying NH4Cl addition or washout, whereas in Cortland buffer, addition of NH4Cl produced a significant relaxation ({dagger}) and washout produced a contraction that was significantly greater than the corresponding contraction in either Hepes or Hepes + HCO3– (*). Values are means ± s.e.m.; Hepes (N=14), Hepes+HCO–3 (N=4), Cortland (N=8).





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