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First published online June 15, 2006
Journal of Experimental Biology 209, 2554-2566 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02259

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Following the heart: temperature and salinity effects on heart rate in native and invasive species of blue mussels (genus Mytilus)

Caren E. Braby^* and George N. Somero

Hopkins Marine Station, Department of Biological Sciences, Stanford University, Oceanview Boulevard, Pacific Grove, CA 93950, USA

Author for correspondence (e-mail: somero{at}stanford.edu)

Accepted 11 April 2006

	Summary

TOP Summary Introduction Materials and methods Results Discussion References

 
The three species of blue mussels, Mytilus trossulus Gould 1850, M. edulis Linnaeus 1758 and M. galloprovincialis Lamarck 1819, have distinct global distribution patterns that are hypothesized to reflect differences in their tolerances of temperature and salinity. We examined effects on heart rate (beats min^–1) of acute exposure and acclimation to different combinations of temperature and salinity to test this hypothesis and, in the context of the invasive success of M. galloprovincialis, to gain insights into the factors that may explain the replacement of the temperate Pacific native, M. trossulus, by this Mediterranean Sea invader along much of the California coast. Heart rate of M. trossulus was significantly higher than that of M. galloprovincialis, consistent with evolutionary adaptation to a lower habitat temperature (temperature compensation) in the former species. Heart rates of M. trossulus/M. galloprovincialis hybrids were intermediate between those of the parental species. Following acclimation to 14°C and 21°C, heart rates of all species exhibited partial compensation to temperature.

Heart rate increased with rising temperature until a high temperature was reached at which point activity fell sharply, the high critical temperature (H_crit). H_crit increased with increasing acclimation temperature and differed among species in a pattern that reflected their probable evolutionary adaptation temperatures: M. galloprovincialis is more heat tolerant than the other two congeners. Ability to sustain heart function in the cold also reflected evolutionary history: M. trossulus is more cold tolerant than M. galloprovincialis.

Heart rates for all three congeners decreased gradually in response to acute reductions in salinity until a low salinity (S_crit) was reached at which heart rate dropped precipitously. S_crit decreased with decreasing salinity of acclimation and was generally lowest for M. galloprovincialis. Mortality during acclimation under common garden conditions was greatest in M. trossulus and was highest at high acclimation temperatures and salinities. These intrinsic differences in basal heart rate, thermal and salinity responses, acclimatory capacity, and survivorship are discussed in the contexts of the species' biogeographic patterning and, for the invasive species M. galloprovincialis, the potential for further range expansion along the Pacific coast of North America.

Key words: acclimation, biogeography, invasive species, Mytilus trossulus, Mytilus edulis, Mytilus galloprovincialis, salinity, temperature

	Introduction

TOP Summary Introduction Materials and methods Results Discussion References

 
Determining the roles played by physiological adaptations in establishing biogeographic patterning is an important goal of ecological and evolutionary physiology (Hochachka and Somero, 2002). Identifying the physiological determinants of latitudinal and vertical distribution patterns can not only explain past and present biogeographic patterning, but also assist in formulating predictions about the effects of climate change on biological communities (Parmesan et al., 1999; Roemmich and McGowan, 1995), especially in the case of range expansions and contractions. Similarly, physiological analysis may assist in explaining and predicting the success of introduced species that have entered a new environment due to human activities that have allowed traditional biogeographic barriers to be bypassed, as species are transported beyond their natural ranges (Carlton and Geller, 1993). There are many cases in which these introduced species persist and become invasive in the new habitat, warranting an analysis of the mechanism(s) accounting for successful range expansion and invasion. One hypothesis is that invasive species are physiologically poised to adjust to new habitats by being particularly tolerant of environmental stressors. Here, we explore the effects of acute and acclimatory changes in temperature and salinity on Mytilus blue mussel congeners, including native and invasive species with distinct distribution patterns along the coast of California.

There are three Mytilus blue mussel congeners worldwide: Mytilus trossulus, M. edulis and M. galloprovincialis (McDonald and Koehn, 1988; McDonald et al., 1991). M. trossulus is native to the North Pacific and is thought to have given rise to the latter two species, from the North Atlantic and the North Atlantic/Mediterranean, respectively (Seed, 1992). The blue mussel species hybridize in all known regions of overlap but only M. galloprovincialis has a demonstrated ability to invade novel locations, including South Africa, Japan and California (Seed, 1992). The congeners differ in their expected tolerance to habitat temperature and salinity, based on their speciation pattern and on their current geographic distribution. M. trossulus is thought to be the most tolerant of cold and low salinity conditions as a species that originated in the North Pacific, while M. galloprovincialis is thought to be the most tolerant of warm, high salinity conditions as a species that originated in the Mediterranean (Seed, 1992). The third blue mussel congener, M. edulis, is thought to have evolved from ancestral M. trossulus (via a trans-Arctic migration) and given rise to the Mediterranean population of blue mussels, which evolved into M. galloprovincialis (Barsotti and Meluzzi, 1968; Vermeij, 1991). M. edulis shares latitudinal overlap to the north with M. trossulus (both occur at latitudes >66°N) and with both species to the south (28–35°N) (Hilbish et al., 2000). Because M. galloprovincialis is limited in its northern distribution (current known limit is Great Britain, 55°N), it is thought that this species may be limited by cold conditions.

The California hybrid zone between M. trossulus and M. galloprovincialis was only recently described (McDonald and Koehn, 1988; McDonald et al., 1991) and thus has been the subject of relatively few studies. This zone of secondary contact was created by the introduction of M. galloprovincialis to Southern California, presumably by shipping, in the first half of the twentieth century (Geller, 1999; McDonald and Koehn, 1988). M. galloprovincialis has replaced M. trossulus over a large fraction of the California coast, from the Mexican border to the approximate latitude of Monterey (37°N). In the Monterey and San Francisco Bays, the native and invader coexist and are found in a mosaic hybrid zone that comprises habitats differing widely in temperature and salinity (Braby and Somero, 2006). Although Mytilus blue mussels occur commonly on the open coast of both the North Atlantic and parts of the North Pacific (in addition to other geographic regions), they are rare in open coast habitats in California, and abundant only in protected, often estuarine, sites. In these protected sites, the mosaic hybrid zone pattern of Mytilus species is hypothesized to be evidence of physiological adaptation to local abiotic environmental conditions (Harrison and Rand, 1989; Sarver and Foltz, 1993; Rawson et al., 1999). In support of this conjecture there is evidence that adult distribution correlates with temperature and salinity gradients (Sarver and Foltz, 1993; Suchanek et al., 1997; Rawson et al., 1999; Braby, 2004; Braby and Somero, 2006). It is suggested that M. trossulus is less tolerant of high temperature and more tolerant of both low salinity and low temperature, consistent with the evolutionary history of the species. It is known that Mytilus congeners have different tolerances of high temperature and that thermal tolerance changes with acclimation/acclimatization history (Hofmann and Somero, 1996; Roberts et al., 1997; Buckley et al., 2001). However, these limited data on thermal physiology do not provide an adequate basis for explaining the distribution patterns of the blue mussels or for predicting the future course of the invasion by M. galloprovincialis.

Even less is known about the importance of salinity effects on the distribution of these species. There is some evidence that M. trossulus may be more euryhaline than the other blue mussel congeners, although results to date are equivocal. Thus, although laboratory experiments comparing growth rates and feeding in adult M. trossulus and M. edulis across salinity treatments found no difference between the congeners' performance (Gardner and Thompson, 2001), differences in larval survival rates of the two species suggest that M. trossulus is more euryhaline (Qiu et al., 2002). However, no physiological studies have addressed the question of whether M. trossulus and M. galloprovincialis respond differently to changes in salinity.

To elucidate the roles of temperature and salinity in establishing the distribution patterns of blue mussels, we investigated the effects of acute and acclimatory changes in temperature and salinity on heart function in intact, immersed animals. Heart rate has been used successfully as a proxy for whole-animal stress in Mytilids in response to toxins (Davenport, 1977; Grace and Gainey, 1987; Depledge et al., 1996; Wedderburn et al., 2000), temperature (Pickens, 1965; Helm and Trueman, 1967; Coleman and Trueman, 1971), salinity (Stickle and Sabourin, 1979; Nicholson, 2002) and predation (Rovero et al., 1999). Here, we demonstrate that significant differences in the responses of the three blue mussel congeners to variations in temperature and salinity may account, at least in part, for their different distribution patterns and may help to explain and predict the invasive success of M. galloprovincialis.

	Materials and methods

TOP Summary Introduction Materials and methods Results Discussion References

 
Animal collection and care
We collected blue mussels from protected, subtidal habitats (floating docks in harbors) whose temperatures and salinities could be readily measured (Braby and Somero, 2006). Locally collected specimens (Monterey Bay and San Francisco Bay mosaic hybrid zone) were brought to the Hopkins Marine Station in coolers within 24 h of collection, and then transferred to closed-circulation, aerated, and filtered 75 l seawater aquaria. Because the congeners and their hybrids can only be distinguished by genetic methods (see below), we also collected animals from blue mussel populations outside of the California hybrid zone in order to ensure adequate and equal numbers of each species in our studies. We received animals within 36 h of collection in moist (but not immersed) conditions from four locations: Coos Bay, Oregon (43°N; Mytilus trossulus Gould); Morro Bay (35°N) and Santa Barbara, California (34°N; Mytilus galloprovincialis Lamarck); and, for experiments with the Atlantic congener, Narragansett Bay, RI (41°N; Mytilus edulis Linnaeus). After size sorting animals (retaining those within the 30–60 mm range) and cleaning encrusting organisms from the shells, we marked the shells with nail lacquer, so that all species could be acclimated in common-garden tanks. Each week, we fed animals 2–3 times with commercially available phytoplankton mix (Reed Mariculture, Shellfish Diet – 1800 Formula, Campbell, CA, USA) and also exchanged half the tank volume with filtered seawater each week, cleaning out accumulated pseudofeces. With the exception of acclimation treatments (described below), we held animals at 13–14°C and 28 p.p.t. salinity (obtained by diluting filtered Monterey Bay seawater with deionized water).

View larger version (49K): [in this window] [in a new window]   Fig. 1. Heart rate response to temperature and methods for impedance pneumography. Heart activity patterns from a single individual over a range of temperatures from 14.5–31°C are shown. Each heart rate trace spans 3 min. Inset shows the schematic of the heart rate monitoring apparatus, and the placement of electrodes. Mussel drawing adapted from (Brusca and Brusca, 1990).

Temperature stress and heart rate
To test the response of heart rate to simulated diel water temperature fluctuations, we used increasing temperature ramps at a rate of change of 6°C h^–1, somewhat faster than the thermal changes seen in blue mussel habitat during the summer months (maximum rate +1.6°C h^–1) (Braby, 2004). Each experiment consisted of a 1 h recovery period at the acclimation temperature (to allow the animals to recover from the electrode implantation procedure), followed by an increase in temperature (1°C per 10 min interval), while salinity was held constant at the acclimation level. Temperature increases continued until all individuals showed a significant drop in heart rate (the critical temperature, H_crit) (Fig. 2A), at which point we stopped the temperature ramp, and decreased the temperature back to the acclimation (initial) value. All animals that exhibited a stable basal heart rate and a characteristic heat ramp response recovered from the heat stress. A small subset of individuals (5 of 89 specimens) was not included in the analysis because the heart activity of these individuals appeared erratic from the beginning of the experiment.

View larger version (18K): [in this window] [in a new window]   Fig. 2. Environmental exposure and heart rate response to stress in M. trossulus. (A) Heat stress; as temperature increases (broken line; beginning at 01:00 h, experimental time), heart rate increases until the animal reaches a temperature at which heart rate falls rapidly (Hcrit). With continued heat stress, heart rate remains depressed, but as soon as temperature drops, heart rate returns to a normal level. (B) Cold stress; as temperature decreases from acclimation level, heart rate decreases to zero. (C) Low salinity stress; as salinity decreases from acclimation level, heart rate decreases very gradually until the animal reaches a salinity at which there is a rapid fall in heart rate (Scrit). Heart rate remains depressed during continued low salinity treatment but recovers immediately when salinity begins to increase.

Salinity stress and heart rate
We used decreasing salinity ramps to test the response of heart rate to salinity fluctuations, as seen in subtidal habitats during periods of spring tides and heavy winter rain run-off (Fig. 2C) (Braby and Somero, 2006). Each experiment consisted of a 1 h post-surgical recovery period at the acclimation temperature, followed by a decrease in salinity (2–3 p.p.t. h^–1), while temperature was held constant at the acclimation level. We decreased chamber salinity by pumping in low salinity water through a peristaltic pump, while an overflow valve kept the volume of the experimental chamber constant. Each experiment continued until all individuals showed a significant drop in heart rate (critical salinity, S_crit), at which point we stopped the salinity ramp. All animals that exhibited a stable heart rate at the beginning of the experiment survived the salinity stress.

Effects of thermal and salinity acclimation on heart rate
During winters of 2003 and 2004, we conducted multi-factor acclimations with six acclimation tanks in each year: three salinities (22 p.p.t., 28 p.p.t. and 34 p.p.t. in both years) and two temperatures (14°C and 21°C in 2003; 7°C and 14°C in 2004). These temperatures and salinities are conditions that the mussels regularly encounter in the field in the California hybrid zone (Braby and Somero, 2006). Two large temperature-controlled aquaria (550 l each) served as water baths and held the three smaller acclimation tanks (75 l) with the salinity treatments. Each acclimation tank had its own filtration/aeration unit and we exchanged half the tank volume each week with temperature-equilibrated seawater of the appropriate salinity (from a mixture of filtered seawater and deionized water or InstantOcean^® supersaturated seawater). The acclimation protocol was as follows: (1) animals were initially held at 14°C and at their collection salinity for 2 days; (2) acclimation tank salinity was changed by 1 p.p.t. day^–1 until it reached the target acclimation salinity; (3) temperature was changed by 2°C day^–1 until it reached the target acclimation temperature. Using temperature data loggers (Dallas Semiconductor, iButtons with ±0.5°C accuracy, Dallas, TX, USA) and a salinity refractometer (Reichert, temperature compensated, Depew, NY, USA), we monitored the temperature and salinity every 2 days and adjusted these as needed. Acclimation conditions stayed within ±1°C and ±1 p.p.t. of the targeted temperatures and salinities throughout the acclimation period. Every 2 days during the acclimation period, we fed animals commercially available phytoplankton mix (Reed Mariculture, Shellfish Diet – 1800 Formula). During the experimental period, we fed animals on a daily basis to eliminate any potential experimental differences due to nutritional state. We began each acclimation period with 30 individuals of each species (a total of 90 individuals per tank in 2002–3 and a total of 60 animals per tank in 2003–4) and we acclimated them for 3 weeks before beginning experiments. On each experiment day, we removed six animals from one acclimation tank for experimental purposes, as well as any sick (gaping) or dead animals. No effect of holding (acclimation) time on heart rates or critical temperatures or salinities was observed.

Data analysis
Because of the high variability of mussel heart beat amplitude over the course of an experiment (Fig. 1), we were unable to use the PowerLab software directly to calculate heart rate throughout the experiments. Thus, setting an arbitrary threshold for amplitude failed to capture the effects of treatments on beats min^–1. Instead, we extracted heart rate manually by selecting the time period of interest and visually counting beats for the selected period. We estimated beats min^–1 for 1 min time intervals, selecting once every 5 min for heating ramps, once every 10 min for cooling ramps and once every 20 min for salinity ramps. When the animal approached its temperature or salinity limit, we calculated beats min^–1 for every 1 min. For heat and salinity ramps, we mathematically derived critical values by finding the common solution of the best-fit lines before and after the distinct shift in heart rate (Stillman and Somero, 1996). When challenging animals with low temperature stress, we calculated the lowest heart rate (LHR) during the 1 h holding period near 0°C.

We evaluated the effects of treatments on these three response variables (H_crit, S_crit and LHR) by applying a multiplicative three-factor ANOVA model with species, acclimation temperature, and acclimation salinity as the three factors. We followed the ANOVAs with post-hoc multiple comparisons (Student–Newman–Keuls, P=0.05), which compared species at each of the temperature/salinity combinations.

Genetic identification
Congeners in the Mytilus blue mussel complex are difficult to distinguish because of their morphological similarity, so we genotyped all the animals used in these experiments. We dissected animals after each experiment, and during the 2003 experiments made qualitative notes of the reproductive status of each individual (M. trossulus and M. galloprovincialis were developed and M. edulis was not). We used multiple DNA isolation protocols throughout the course of these experiments, including membrane spin columns (Macherey-Nagel, Nucleospin^® DNA extraction kit, Easton, PA, USA), guanidinium salt and silica bead extraction (Hoss and Paabo, 1993), and Proteinase K tissue digestion [10 mmol l^–1 Tris-HCl pH 8, 1 mmol l^–1 EDTA, 0.3% Tween, 0.3% nonylphenyl-polyethylenglycol (Sigma, IGEPAL CA-630), 0.03 units µl^–1 Proteinase K; 55°C for 12 h, 98°C for 10 min]. We used previously described polymerase chain reaction (PCR)-based methods to amplify two nuclear loci: the byssal thread protein locus (Glu-5') (Rawson et al., 1996) and the ribosomal internal transcribed spacer region (ITS-1) (Heath et al., 1995).

The Glu-5' locus amplifies a different sized fragment for M. galloprovincialis (300 bp), M. edulis (350 bp) and M. trossulus (240 bp), because of an insertion in the M. galloprovincialis gene and a double insertion in the M. edulis gene. An F1 M. trossulus/M. galloprovincialis hybrid (T/G hybrid) amplifies both sized fragments (240 bp and 300 bp). We used the published primers (F-GTAGGAACAAAGCATGAACCA; RGGGGGGATAAGTTTTCTTAGG), slightly modified PCR chemistry (15 µl reaction volume with 1 µl DNA template, 0.1 Units Taq polymerase, 1x Taq Buffer [50 mmol l^–1 KCl, 30 mmol l^–1 Tricine, pH 8.6], 200 nmol l^–1 dNTPs, 2 mmol l^–1 MgCl₂, 300 nmol l^–1 of each primer) and slightly modified cycling conditions (initial denaturation of 1.5 min at 94°C; 35 cycles of 94°C for 20 s, 53°C for 30 s and 72°C for 45 s; final extension of 2 min at 72°C).

The ITS-1 locus amplifies a similar sized fragment in all three species (950 bp), but M. trossulus can be distinguished from M. galloprovincialis/M. edulis by the number of recognition sites for the HhaI restriction enzyme. After a restriction digest of the PCR products, there are several fragments but two unique fragment sizes (M. trossulus= 280 bp; M. galloprovincialis/M. edulis=450 bp). We used the published primers (F-GTTTCCGTAGGTGAACCTG; RCTCGTCTGATCTGAGGTCG), slightly modified PCR chemistry (same recipe as for Glu-5', described above) and slightly modified cycling conditions (initial denaturation of 1.5 min at 94°C; 30 cycles of 94°C for 20 s, 55°C for 30 s and 72°C for 1 min; final extension of 2 min at 72°C). To digest the PCR product, we then added 0.2 µl HhaI enzyme (New England Biolabs, Ipswich, MA, USA), 1.5 µl 10x buffer (New England Biolabs–Buffer 4) and 0.15 µl bovine serum albumin (100 µg ml^–1) to each PCR reaction and digested for 4 h at 37°C.

We visualized PCR products for both loci using gel electrophoresis on a 2% agarose gel, stained with ethidium bromide. To arrive at a final genotype designation, we scored individuals at each locus separately and then scored across both loci. These final designations are: M. galloprovincialis, M. trossulus, T/G hybrid (potentially includes backcrossed genotypes), and M. edulis.

View larger version (28K): [in this window] [in a new window]   Fig. 3. Heart rate response to temperature and salinity in differently acclimated mussels. Species are referred to by the first letter of the species' name: M. trossulus (t), M. galloprovincialis (g), M. edulis (e), and M. trossulus/M. galloprovincialis hybrids (h). Symbols connected by solid lines are 14°C-acclimated; symbols connected by dotted lines are 21°C-acclimated. Values are means ± s.e.m. For N and P values, see Table 2. (A) Resting heart rate measured at the acclimation temperatures (14°C or 21°C). (B) Response to heat stress (Hcrit); all species show an increase in Hcrit with increased acclimation temperature. While there are some differences in Hcrit among salinity treatments, salinity acclimation does not contribute statistically to the observed pattern. (C) Response to cold stress (lowest heart rate at 0°C); at comparable acclimations, M. trossulus has an equal or higher heart rate than M. galloprovincialis. Note that to make overlapping symbols visible, we offset the g-14°C data by +0.2°C along the x-axis. (D) Response to low salinity stress (Scrit); there is a clear increase in Scrit with increasing salinity acclimation in all species.

	Results

TOP Summary Introduction Materials and methods Results Discussion References

Heat stress and heart rate
In response to acute increases in measurement temperature, heart rate initially increased and, then, abruptly decreased when the critical high temperature (H_crit) was reached (Fig. 2A). During continued exposure to temperatures >H_crit, heart rate remained depressed but quickly returned to the initial rate when the water bath was returned to the initial experimental temperature (=acclimation temperature; see Fig. 2A). This indicates that the pronounced fall in heart rate that occurs when H_crit is reached is not lethal, but rather is a temporary response to an elevated environmental temperature and is likely coincident with valve closure (see Discussion).

Significant differences were observed in comparisons of the different species and, within a species, of differently acclimated individuals. All species exhibited increases in H_crit as acclimation temperature was increased (Fig. 3B) (ANOVA, P<0.0001, Tables 1, 2). The combination of acclimation temperature and species explained a significant portion of the variation seen in H_crit (ANOVA, P<0.0001, Tables 1, 2), but salinity acclimation was not a significant contributor to the observed pattern (P=0.7791).

Although not all interspecific comparisons were statistically significant at each temperature/salinity combination, there are consistent trends in H_crit among species that suggest that the congeners respond differently to heat stress (Fig. 3B). Mytilus trossulus had the lowest H_crit and so appears to be the most sensitive to heat stress, followed by M. edulis, and M. galloprovincialis. These relationships among the three species do not vary as a function of temperature acclimation. At the one acclimation treatment so studied (14°C, 28 p.p.t.), M. trossulus/M. galloprovincialis hybrids had H_crit values intermediate to those of the parental species. Depending on acclimation salinity, M. trossulus increased its H_crit by 1.8–3.4°C during acclimation to 21°C. Mytilus galloprovincialis and M. edulis showed slightly greater acclimatory plasticity as they increased H_crit by 2.4–5.2°C and 2.0–4.7°C, respectively, during acclimation at 21°C.

Cold stress and heart rate
Heart rate fell steadily with decreasing measurement temperature, but no sharp breaks in cardiac activity such as those seen during heating were observed (Fig. 2A,B). After cold stress and a return to the acclimation temperature, the heart rates of both species tested (M. trossulus and M. galloprovincialis) quickly recovered to values seen at the start of the experiment (acclimation temperature). Comparing the lowest heart rates measured near 0°C (LHR) (Fig. 3C), it is clear that M. trossulus was able to maintain heart function at significantly lower temperatures than M. galloprovincialis (ANOVA, P<0.0001, Tables 1, 2) under most acclimation conditions. Both species showed significant acclimation effects. The heart rate of 14°C-acclimated animals was severely depressed by cold stress, with a measurable heart rate only in M. trossulus and only at a single salinity (28 p.p.t.). Following acclimation to 7°C, an increase in heart rate of up to 6 beats min^–1 was observed in M. trossulus, but for M. galloprovincialis heart rate increased by only 2 beats min^–1. At common winter temperatures, therefore, the heart rate of M. trossulus would exceed that of M. galloprovincialis by at least two- to threefold, depending on salinity.

Salinity stress and heart rate
Heart rate decreased gradually as the salinity of the medium was reduced and exhibited a characteristically sharp drop when salinity reached a certain low value, the critical low salinity (S_crit) (Fig. 2C). S_crit was positively correlated with acclimation salinity in all species (Fig. 3D; Tables 1, 2). In addition, S_crit was positively correlated with acclimation temperature in some treatments. Acclimation to a higher temperature increased S_crit of M. trossulus and M. edulis (Fig. 3D, ANOVA, P<0.0001, Tables 1, 2). Of all the species, M. edulis was most affected by the interaction of warm temperature acclimation and the response of heart rate to salinity, raising its S_crit by 4–9 p.p.t., depending on the salinity of acclimation. In contrast, M. galloprovincialis had a similar S_crit, regardless of the acclimation temperature. Among the congeners, heart rate of M. galloprovincialis is sustained at the lowest ambient salinities, that of M. edulis ceases at the highest S_crit, and M. trossulus is intermediate. Pilot studies showed that M. trossulus/M. galloprovincialis hybrids were not different in their salinity tolerance in comparison to the three congeners at moderate acclimation levels (14°C and 28 p.p.t.) (S_crit11.8 p.p.t.).

Q₁₀ and temperature compensation of heart rate during thermal acclimation
To determine whether temperature compensation of heart rate occurred during thermal acclimation (14°C and 21°C), we calculated the Q₁₀ of heart rate of 14°C-acclimated individuals (each species), using data from the initial hour of heat ramps. The Q₁₀ values differed only slightly among species (M. galloprovincialis=2.38, M. trossulus=2.03, M. edulis=2.08). We then used these Q₁₀ values to predict heart rate at the higher acclimation temperature (21°C), in the absence of temperature compensation. We calculated that we should see a 60–70% increase in heart rate during acclimation from 14°C to 21°C, if there were no temperature compensation. In fact, we observed partial temperature compensation due to acclimation, with rate increases of only 35–64% in heart rate (Fig. 4), depending on acclimation treatment.

View larger version (21K): [in this window] [in a new window]   Fig. 4. Temperature compensation of resting heart rate (RHR; beats min–1). t, M. trossulus; g, M. galloprovincialis; e, M. edulis. Symbols connected by solid lines are observed RHR values averaged across salinity treatments. Symbols connected by broken lines are observed RHRs at 14°C and expected RHRs at 21°C, using the species-specific Q10 estimate and assuming no physiological compensation. If there was complete temperature compensation, there would be no difference between the observed values for 14°C- and 21°C-acclimated animals (symbols connected by a horizontal line; not shown). However, the observed RHR at 21°C is lower than expected for all species, indicating partial compensation with acclimation temperature.

View larger version (19K): [in this window] [in a new window]   Fig. 5. Mortality in temperature and salinity acclimation treatment groups. There was nearly equal mortality of M. edulis (e) and M. trossulus (t) in 2003, regardless of acclimation treatment, and both species had greater mortality at the warmer acclimation temperature. In the following year, M. trossulus had equivalent mortality at the 14°C acclimation and decreased mortality under 7°C acclimation, reinforcing the positive relationship between acclimation temperature and mortality. Only three M. galloprovincialis (g) individuals died in both acclimation years (out of 348 total), and all three were from the 21°C acclimation in 2003 (M. galloprovincialis 2004 is not included in the graph because there was no observed mortality).

	Discussion

TOP Summary Introduction Materials and methods Results Discussion References

 
Limits to biogeographic patterns are clearly the result of many ecological, historical, and physiological factors. However, among these factors, we perhaps know the least about the roles of physiological limits in governing distribution ranges. In this manuscript, we present data that enable us to address a number of questions about the roles played by fixed genetic differences in temperature and salinity tolerance and by varying capacities for acclimation to these two variables in setting distribution patterns of closely related blue mussel congeners. The evolutionary history of the blue mussel complex suggests that differences in tolerance of temperature and salinity and in the capacities to acclimatize to changes in both variables should exist among species. The congeners are separated by approximately 3.5–5 million years of evolutionary divergence, yet the two most diverged congeners in the blue mussel complex (M. galloprovincialis is the newest species and M. trossulus the oldest) (Barsotti and Meluzzi, 1968; Seed, 1992; Vermeij, 1991), remain capable of hybridizing in all known regions of contact. However, the habitats in which these two species arose differ in temperature and salinity characteristics, despite sympatric extant populations. The Mediterranean Sea is a warm, enclosed sea with a relatively high and stable salinity, compared to the North Pacific Ocean, which is relatively cool and may have coastal regions of low salinity during periods of run-off from winter storms. From these native habitat differences, one would predict M. trossulus to be successful in habitats with fluctuating salinity and cooler temperatures and M. galloprovincialis to be more successful in habitats with stable and higher salinities and higher temperatures. However, the current global distribution and invasive success of M. galloprovincialis suggest that it may be the most physiologically adaptable of the Mytilus congeners and that it may have fewer physiological limitations to range expansion than its congeners.

Thermal physiology
Habitat temperature is often cited as a limiting factor determining where ectothermic species can live (Hochachka and Somero, 2002). Because water temperature and blue mussel species composition were highly variable among the sites we examined (Braby, 2004; Braby and Somero, 2006), any observed differences between M. trossulus and M. galloprovincialis in their responses to temperature could provide a mechanistic explanation for the distribution of these species within the mosaic hybrid zone present in San Francisco and Monterey Bays. Previous work (Sarver and Foltz, 1993) on the distributions of these two congeners in this hybrid zone suggested that M. trossulus was better adapted to conditions of low temperature and low salinity than M. galloprovincialis. However, this earlier study did not examine physiological traits, but focused strictly on the distributions of the two species.

Our study provides evidence for genetically fixed differences in the thermal responses of heart function among the three blue mussel congeners, differences that may account, in part, for their current biogeographic patterning and, in the context of predicting the further invasive success of M. galloprovincialis, for the ability of this invader to extend its range northward. Mytilus trossulus is more cold tolerant than the invasive M. galloprovincialis, as evidenced by its capacity for sustaining cardiac function at low temperatures (Fig. 3C). Under winter conditions, cardiac activity would be substantially lower in the invasive species than in M. trossulus, a physiological difference that could favor the native species at higher latitudes, where winter temperatures during emersion may reach 0°C. Likewise, our discovery of a significantly higher intrinsic rate of cardiac activity in M. trossulus relative to M. galloprovincialis (Fig. 3A) suggests that the native species is more cold-adapted (cold-compensated) than the Mediterranean invader. Our data on heart rates provide an interesting complement to an earlier study of blue mussels (Pickens, 1965), which was conducted before it was known that the blue mussels found along the Pacific Coast of North America were not M. edulis, but instead comprised M. trossulus, M. galloprovincialis and their hybrids. Pickens reported that `M. edulis' populations from Alaska (putatively, M. trossulus) had significantly higher heart rates than `M. edulis' populations from Southern California (putatively, M. galloprovincialis). We conjecture, therefore, that replacement of M. trossulus by M. galloprovincialis at higher latitudes may be impeded by the native species' better ability to sustain cardiac function at colder temperatures.

Other studies support the view that M. trossulus has a more cold-adapted physiology than M. galloprovincialis, and, therefore, would be at a competitive advantage at low, but not high, temperatures. A recent study (Fields et al., 2006) compared the thermal sensitivities of cytosolic malate dehydrogenase (cMDH) of M. trossulus and M. galloprovincialis and showed that the cMDH of M. trossulus exhibited kinetic properties consistent with adaptation to lower temperatures relative to the ortholog of M. galloprovincialis. Hofmann and Somero provide additional evidence that M. trossulus is the more cold-adapted species (Hofmann and Somero, 1996), through showing that M. galloprovincialis induces synthesis of heat-shock proteins at a higher temperature than M. trossulus (in 13°C-acclimated animals, induction temperatures were 25°C and 23°C, respectively). The greater tolerance of high temperatures by M. galloprovincialis, e.g. the higher values for H_crit (Fig. 3B), and the much lower mortality found at the highest acclimation temperature (21°C) (Fig. 5) indicate that this invasive species is likely to have a competitive advantage over the native under conditions of high temperature. These differences could underlie the success of the invader in replacing M. trossulus along much of the coastline of southern and central California.

Although M. trossulus appears better adapted, physiologically and biochemically, than M. galloprovincialis to function at low temperatures and thus seems poised to out-compete the invader at higher latitudes, the occurrence of higher mortality of M. trossulus under common garden conditions at both low and high temperatures (Fig. 5) raises a caveat about the relative competitive abilities of these two species. Higher mortalities for northern populations of `M. edulis' (likely, M. trossulus) than for southern populations (likely, M. galloprovincialis) have also been reported (Pickens, 1965). Pickens' data and ours suggest that some currently unknown difference in robustness exists between these two species, and these differences in adult survivorship could potentially balance or even outweigh the competitive differences due to differential adaptations to temperature at the physiological and biochemical level.

Another caveat about the significance of physiological adaptations also merits consideration. The sharp decreases in cardiac activity seen at extremes of temperature may be a consequence of a complex behavioral response to stress, rather than merely the direct effects of temperature on the heart itself. Pickens compared the effects of temperature on hearts in situ and on hearts isolated from the mussels (Pickens, 1965). Hearts under both experimental conditions exhibited a similar Q₁₀ effect as measurement temperature was varied. However, the isolated hearts sustained function at higher and lower temperatures than hearts in situ. The differences in the thermal responses between these two experimental preparations may arise from valve closure. A few studies have quantified valve closure response to toxins (Depledge et al., 1996) and to salinity stress (Nicholson, 2002) in Mytilid mussels. We qualitatively demonstrated that temperatures near H_crit elicited valve closure (Braby, 2004); however, this was done after the experiments reported in this paper were completed. Thus, the sharp fall in heart rate seen at temperature extremes in intact animals could be a consequence of a behavioral response to stress, valve closure, which in turn elicits physiological adjustments. Closing of the valves would restrict gas exchange, thus limiting aerobic metabolism. Under conditions of limiting oxygen, reductions (or cessations) of cardiac activity might be necessary. In the context of thermal effects, valve closure responses could be especially advantageous during periods of emersion, when the threat of desiccation at high temperatures may be significant. Future research should quantify valve closure in response to temperature (and salinity) stress and to determine which comes first, valve closure or heart rate depression.

Salinity physiology
Valve closure, which we assume is coupled with a reduction in cardiac activity under all circumstances, could also be important for immersed animals facing sharp reductions in salinity, as might occur during winter rainstorms in the northeastern Pacific. For osmoconformers like Mytilus, reductions in ambient salinity will lead to dilution of the internal fluids and perturbation of the ionic compositions of the cells. In Mytilus, the physiological response to chronic hypo-osmotic conditions is to actively change the cytosolic concentration of various organic osmolytes, including free amino acids (Yancey et al., 1982), which may require 2 or more days to achieve (de Vooys, 1991; Gosling, 1991; Hawkins and Bayne, 1991). However, in response to acute hypo-osmotic stress, conservation of the composition of the body fluids would be favored by closing the valves and, thereby, reducing exchange of water and solutes with the ambient seawater. In the case of acute hypo-osmotic stress, therefore, it may be advantageous to close the valves (and, consequently, reduce cardiac activity) at relatively high salinities. Thus, a high S_crit might be advantageous in coping with hypo-osmotic stress, for this would allow a mussel to isolate its body fluids from falling salinity at an earlier stage of stress exposure. Earlier studies have, in fact, shown that acute fluctuations in salinity trigger valve closure in mussels (de Vooys, 1991; de Zwaan and Mathieu, 1991; Seed and Suchanek, 1991).

Viewed from this perspective, the higher S_crit found for M. trossulus relative to M. galloprovincialis might represent an adaptation for coping with hypo-osmotic stress, which is apt to be more common in coastal Pacific habitats than in the Mediterranean Sea. If this is the case, then another difference between the native and invasive species appears to be important in determining their biogeographic patterns and in determining the further invasive movement of M. galloprovincialis. The greater ability of M. trossulus to isolate its internal fluids from hypo-osmotic stress could help it to out-compete M. galloprovincialis in habitats where such stress is prevalent. Especially during winter storms, when the combination of low temperatures and reduced salinity exists in coastal regions, M. trossulus might be significantly better able to function than its invasive competitor. We conjecture that the reduction in feeding time and the depression in metabolic activity that are likely to result from valve closure are more than balanced by the reduction in physiological stress associated with volume regulation due to hypo-osmotic conditions.

S_crit varied directly with acclimation salinity, such that animals held at the high salinity reduced cardiac activity at higher salinities than specimens acclimated to lower salinities (Fig. 3D). Acclimation temperature also influenced S_crit. For M. trossulus, S_crit is lower in 14°C-acclimated specimens relative to 21°C-acclimated specimens (Fig. 3D). This finding suggests that winter-acclimatized individuals may be better able to cope with reduced salinities than summer-acclimatized individuals. There was relatively little change in S_crit during thermal acclimation in M. galloprovincialis. M. edulis exhibited the largest changes in S_crit as functions of acclimation conditions. Our experiments did not monitor the time course of the change in S_crit during acclimation, but a recent study shows that cyclical hypo-osmotic stress confers greater salinity tolerance with each cycle (quantified by valve closure) (Sukhotin et al., 2003) and that this increased tolerance occurs over the course of a few hours.

Hybrid physiology
Hybrids between M. trossulus and M. galloprovincialis exhibited intermediate values for resting heart rate (Fig. 3A) and H_crit (Fig. 3B). S_crit values fell within the range of the parental species (Fig. 3D). These limited data provide no evidence for any distinct advantage or disadvantage on the part of adult hybrids. However, there is some indication from reproductive studies that larval success of hybrid crosses of these two species is lower than for parental species (Matson et al., 2003).

What is the most critical physiological factor in setting distribution patterns of blue mussels?
Our studies of adult blue mussels reveal a number of physiological differences that correlate with the species' distribution patterns. M. galloprovincialis is better able to cope with higher temperatures than either M. trossulus or M. edulis. Assuming that a high value for S_crit, which is likely paired with valve closure, denotes an adaptive response to hypo-osmotic stress, then M. trossulus appears better adapted than its invasive competitor to tolerate reduced salinity. These differences between M. trossulus and M. galloprovincialis in tolerance of thermal and salinity extremes are consistent with the evolutionary histories of the two species. However, despite this agreement between physiological response and evolutionary history, two important questions about the role of these physiological differences in setting distribution patterns remain. First, which environmental variable is more important in setting distributions, temperature or salinity? Second, do the physiological data obtained in this study reflect the distribution patterns of the native and invasive species within the mosaic hybrid zone found in the San Francisco and Monterey Bays?

Based on our study of the distributions of the native and invasive species within this mosaic hybrid zone (Braby and Somero, 2006), it appears that salinity is the more important variable in the context of determining the distribution of species. Thus, M. trossulus was found to be dominant in the warmest habitats studied, which are shallow estuarine regions with the highest variation in salinity.

Adult distribution patterns may also reflect differences in larval recruitment over space and time and different sensitivities of congeneric larvae to environmental factors. Other studies of Mytilus have shown that the larvae of Mytilus congeners have differences in mortality due to salinity and temperature treatments (Matson et al., 2003; Qiu et al., 2002). In the case of the two Pacific coast species used in this study, there is only limited knowledge of where larvae spend most of their planktonic life. In the open ocean, there is likely to be little variation in temperature and salinity. The degree to which larvae are subject to coastal variability in both temperature and salinity is highly dependent on their retention in bays and estuaries. If high retention occurs, then larval physiology may play a significant role in determining adult distribution patterns. In fact, there is some evidence that Mytilus may be recruiting more locally than previously thought (Gilg and Hilbish, 2003). Nonetheless, the differences in adults' responses to temperature and salinity reported here suggest that genetically fixed differences in physiological traits–differences that are likely present in larval stages as well–make a strong contribution to distribution patterns, including those of invasive species.

	Acknowledgments

 
This publication was supported by (1) the National Sea Grant College Program of the U.S. Department of Commerce's National Oceanic and Atmospheric Administration under NOAA Grant no. NA06RG0142, project no. RC/Z-179, through the California Sea Grant College Program, and in part by the California State Resources Agency; (2) the Partnership for Interdisciplinary Study of the Coastal Oceans (PISCO) (this is PISCO contribution number 214), (3) National Science Foundation grant IBN-0133184, and (4) the Earl and Ethel Myers Marine Biological and Oceanographic Trust. Dr Jonathon Stillman provided technical training and assistance for the impedance pneumography technique. A generous group of colleagues helped collect animals for this study: Maxine Chaney, Dr Gretchen Hofmann, James Lopez, Michelle Phillips, Chip Rerig, Dr Eric Sanford and Dr Allison Whitmer.

	Footnotes

 
^* Present address: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, CA 95039, USA

	References

TOP Summary Introduction Materials and methods Results Discussion References

 

Barsotti, G. and Meluzzi, C. (1968). Osservazioni su Mytilus edulis L. e Mytilus galloprovincialis Lam. Conchiglie 4, 50-58.

Braby, C. E. (2004). Physiological ecology of native and invasive blue mussels (Genus Mytilus) in Central California. PhD thesis, Department of Biological Sciences, Stanford University.

Braby, C. E. and Somero, G. N. (2006). Ecological gradients and relative abundance of native (Mytilus trossulus) and invasive (M. galloprovincialis) blue mussels in the California hybrid zone. Mar. Biol. 148,1249 -1262.[CrossRef]

Brusca, R. C. and Brusca, G. J. (1990). Invertebrates. Sunderland, MA: Sinauer Associates.

Buckley, B. A., Owen, M. E. and Hofmann, G. E. (2001). Adjusting the thermostat: the threshold induction temperature for the heatshock response in intertidal mussels (genus Mytilus) changes as a function of thermal history. J. Exp. Biol. 204,3571 -3579.

Carlton, J. T. and Geller, J. B. (1993). Ecological roulette: the global transport of nonindigenous marine organisms. Science 261,78 -82.[Abstract/Free Full Text]

Coleman, N. and Trueman, E. R. (1971). The effect of aerial exposure on the activity of the mussels Mytilus edulis (L.) and Modiolus modiolus (L.). J. Exp. Mar. Biol. Ecol. 7,295 -304.[CrossRef]

Davenport, J. (1977). A study of effects of copper applied continuously and discontinuously to specimens of Mytilus edulis (L) exposed to steady and fluctuating salinity levels. J. Mar. Biol. Assoc. UK 57, 63-74.

de Vooys, C. G. N. (1991). Anaerobic metabolism in sublittoral living Mytilus galloprovincialis in the Mediterranean IV: role of amino acids in adaptation to low salinities during anaerobiosis and aerobiosis. Comp. Biochem. Physiol. 100A,423 -432.[CrossRef][Medline]

de Zwaan, A. and Mathieu, M. (1991). Cellular biochemistry and endocrinology. In The Mussel Mytilus: Ecology, Physiology, Genetics and Culture (ed. E. Gosling), pp.223 -307. New York: Elsevier.

Depledge, M. H., Lundebye, A. K., Curtis, T., Aagaard, A. and Andersen, B. B. (1996). Automated interpulse-duration assessment (AIDA): a new technique for detecting disturbances in cardiac activity in selected macroinvertebrates. Mar. Biol. 126,313 -319.[CrossRef][Medline]

Fields, P. A., Rudomin, E. L. and Somero, G. N. (2006). Temperature adaptation of cytosolic malate dehydrogenases from native and invasive species of marine mussels (genus Mytilus): implications for biogeographic patterning and invasive success. J. Exp. Biol. 209,656 -667.[Abstract/Free Full Text]

Gardner, J. P. A. and Thompson, R. J. (2001). The effects of coastal and estuarine conditions on the physiology and survivorship of the mussels Mytilus edulis, M. trossulus and their hybrids. J. Exp. Mar. Biol. Ecol. 265,119 -140.[CrossRef][Medline]

Geller, J. B. (1999). Decline of a native mussel masked by sibling species invasion. Conserv. Biol. 13,661 -664.[CrossRef]

Gilg, M. R. and Hilbish, T. J. (2003). The geography of marine larval dispersal: coupling genetics with fine-scale physical oceanography. Ecology 84,2989 -2998.[CrossRef]

Gosling, E. (1991). Genetics of Mytilus. In The Mussel Mytilus: Ecology, Physiology, Genetics and Culture (ed. E. Gosling), pp.309 -382. New York: Elsevier.

Grace, A. L. and Gainey, L. F. (1987). The effects of copper on the heart rate and filtration rate of Mytilus edulis.Mar. Pollut. Bull. 18,87 -91.[Medline]

Harrison, R. G. and Rand, D. M. (1989). Mosaic hybrid zones and the nature of species boundaries. In Speciation and its Consequences (ed. D. Otte and J. A. Endler), pp.111 -133. Sunderland, MA: Sinauer Associates.

Hawkins, A. J. S. and Bayne, B. L. (1991). Physiological interrelations, and the regulation of production. In The Mussel Mytilus: Ecology, Physiology, Genetics and Culture (ed. E. Gosling), pp. 171-222. New York: Elsevier.

Heath, D. D., Rawson, P. D. and Hilbish, T. J. (1995). PCR-based nuclear markers identify alien blue mussel (Mytilus spp.) genotypes on the west coast of Canada. Can. J. Fish. Aquat. Sci. 52,2621 -2627.[CrossRef]

Helm, M. M. and Trueman, E. R. (1967). Effect of exposure on heart rate of mussel Mytilus edulis L. Comp. Biochem. Physiol. 21,171 -177.[CrossRef]

Hilbish, T. J., Mullinax, A., Dolven, S. I., Meyer, A., Koehn, R. K. and Rawson, P. D. (2000). Origin of the antitropical distribution pattern in marine mussels (Mytilus spp.): routes and timing of transequatorial migration. Mar. Biol. 136,69 -77.[CrossRef]

Hochachka, P. W. and Somero, G. N. (2002). Biochemical Adaptation: Mechanism and Process in Physiological Evolution. Oxford: Oxford University Press.

Hofmann, G. E. and Somero, G. N. (1996). Interspecific variation in thermal denaturation of proteins in the congeneric mussels Mytilus trossulus and M. galloprovincialis: evidence from the heat-shock response and protein ubiquitination. Mar. Biol. 126,65 -75.[CrossRef][Medline]

Hoss, M. and Paabo, V. (1993). DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 21,3913 -3914.[Free Full Text]

Matson, S. E., Davis, J. P. and Chew, K. K. (2003). Laboratory hybridization of the mussels, Mytilus trossulus and M. galloprovincialis: larval growth, survival and early development. J. Shellfish Res. 22,423 -430.

McDonald, J. H. and Koehn, R. K. (1988). The mussels Mytilus galloprovincialis and Mytilus trossulus on the Pacific coast of North America. Mar. Biol. 99,111 -118.[CrossRef][Medline]

McDonald, J. H., Seed, R. and Koehn, R. K. (1991). Allozymes and morphometric characters of three species of Mytilus in the Northern and Southern Hemispheres. Mar. Biol. 111,323 -334.[CrossRef]

Nicholson, S. (2002). Ecophysiological aspects of cardiac activity in the subtropical mussel Perna viridis (L.) (Bivalvia: Mytilidae). J. Exp. Mar. Biol. Ecol. 267,207 -222.[CrossRef]

Parmesan, C., Ryrholm, N., Stefanescu, C., Hill, J. K., Thomas, C. D., Descimon, H., Huntley, B., Kaila, L., Kullberg, J., Tammaru, T. et al. (1999). Poleward shifts in geographical ranges of butterfly species associated with regional warming. Nature 399,579 -583.[CrossRef]

Pickens, P. E. (1965). Heart rate of mussels as a function of latitude, intertidal height, and acclimation temperature. Physiol. Zool. 38,390 -405.[Abstract/Free Full Text]

Qiu, J. W., Tremblay, R. and Bourget, E. (2002). Ontogenetic changes in hyposaline tolerance in the mussels Mytilus edulis and M. trossulus: implications for distribution. Mar. Ecol. Prog. Ser. 228,143 -152.[CrossRef]

Rawson, P. D., Agrawal, V. and Hilbish, T. J. (1999). Hybridization between the blue mussels Mytilus galloprovincialis and M. trossulus along the Pacific coast of North America: evidence for limited introgression. Mar. Biol. 134,201 -211.[Medline]

Rawson, P. D., Joyner, K. L., Meetze, K. and Hilbish, T. J. (1996). Evidence for intragenic recombination within a novel genetic-marker that distinguishes mussels in the Mytilus edulis species complex. Heredity 77,599 -607.

Roberts, D. A., Hofmann, G. E. and Somero, G. N. (1997). Heat-shock protein expression in Mytilus californianus: acclimatization (seasonal and tidal-height comparisons) and acclimation effects. Biol. Bull. 192,309 -320.[Abstract]

Roemmich, D. and McGowan, J. (1995). Climatic warming and the decline of zooplankton in the California current. Science 267,1324 -1326.[Abstract/Free Full Text]

Rovero, F., Hughes, R. N. and Chelazzi, C. (1999). Cardiac and behavioural responses of mussels to risk of predation by dogwhelks. Anim. Behav. 58,707 -714.[CrossRef][Medline]

Sarver, S. K. and Foltz, D. W. (1993). Genetic population structure of a species' complex of blue mussels (Mytilus spp.). Mar. Biol. 117,105 -112.[Abstract/Free Full Text]

Seed, R. (1992). Systematics, evolution and distribution of mussels belonging to the genus Mytilus: an overview. Am. Malacol. Bull. 9,123 -137.

Seed, R. and Suchanek, T. H. (1991). Population and community ecology of Mytilus. In The Mussel Mytilus: Ecology, Physiology, Genetics and Culture (ed. E. Gosling), pp.87 -169. New York: Elsevier.

Stickle, W. B. and Sabourin, T. D. (1979). Effects of salinity on the respiration and heart rate of the common mussel, Mytilus edulis L, and the black chiton, Katherina tunicata (Wood). J. Exp. Mar. Biol. Ecol. 41,257 -268.[CrossRef]

Stillman, J. H. and Somero, G. N. (1996). Adaptation to temperature stress and aerial exposure in congeneric species of intertidal porcelain crabs (genus Petrolisthes): correlation of physiology, biochemistry and morphology with vertical distribution. J. Exp. Biol. 199,1845 -1855.[Abstract]

Suchanek, T. H., Geller, J. B., Kreiser, B. R. and Mitton, J. B. (1997). Zoogeographic distributions of the sibling species Mytilus galloprovincialis and M. trossulus (Bivalvia: Mytilidae) and their hybrids in the North Pacific. Biol. Bull. 193,187 -194.[Abstract]

Sukhotin, A. A., Lajus, D. L. and Lesin, P. A. (2003). Influence of age and size on pumping activity and stress resistance in the marine bivalve Mytilus edulis L. J. Exp. Mar. Biol. Ecol. 284,129 -144.[CrossRef]

Vermeij, G. J. (1991). Anatomy of an invasion: the trans-Arctic interchange. Paleobiology 17,281 -307.[Abstract]

Wedderburn, J., McFadzen, I., Sanger, R. C., Beesley, A., Heath, C., Hornsby, M. and Lowe, D. (2000). The field application of cellular and physiological biomarkers, in the mussel Mytilus edulis, in conjunction with early life stage bioassays and adult histopathology. Mar. Pollut. Bull. 40,257 -267.[CrossRef]

Yancey, P. H., Clark, M. E., Hand, S. C., Bowlus, R. D. and Somero, G. N. (1982). Living with water stress: evolution of osmolyte systems. Science 217,1214 -1222.[Abstract/Free Full Text]

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