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Fig. 7. Nrf2 nuclear translocation is related to the administered dose of
paracetamol. Mice were treated with the indicated doses of paracetamol. After
1 h they were sacrificed, livers were removed, nuclei prepared and nuclear
proteins extracted. These were separated using SDS–PAGE, alongside a
recombinant mouse Nrf2 positive control, transferred to a nitrocellulose
membrane, probed for Nrf2 using a polyclonal anti-Nrf2 antiserum and
visualised using chemiluminescence. Each data point in the blot and in the
response-curve is obtained from pooled extracts of five animals (+ s.d.).
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