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Fig. 6. Myogenin (myog) expression in embryos of the tiger pufferfish
(Takifugu rubripes) reared at 15°C (open circles), 18°C
(closed circles) and 21°C (closed triangles). The eggs were from a single
cross. mRNA transcripts were measured by qPCR using 18S rRNA as an endogenous
control. The results were normalised against the highest expression value
(21°C, 40 h.p.f.) and plotted against somite interval (development
time/divided by the time to form one somite pair) in an attempt to normalise
developmental stage at the different temperatures. The results represent mean
± s.e.m. of 4 batches of embryos per temperature. From
(Fernandes et al., 2006).