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Fig. 4. Immunolocalisation of Mvl in both the larval and adult alimentary canal.
(AC) Larval anterior midgut. (A) Mvl staining within the larval
anterior midgut. (B) Higher magnification reveals punctuate subcellular
staining within enterocytes (arrow). (DF) Mvl-positive staining within
the adult posterior midgut. (D) Staining within these enterocytes appears to
be diffuse (arrow), with some punctate staining. (E) Small scattered cells
show strong Mvl staining (arrow). (GI) Mvl localisation within the
Malpighian tubules. (G) Diffuse staining within principal (type 1) cells
(arrow). (H) Strong Mvl-positive staining within small neuroendocrine-like
cells (arrow). (C,F,I) Mvl peptides confirm staining specificity in each cell
type. Scale bars, 200 µm (A,C); 100 µm (B,DI).