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Fig. 1. Reactivity of anti-Mvl antiserum and demonstration of the specificity of
blocking peptides. Western blots of purified wild-type GST, Mvl expressed in
Cos-1 cells by transient transfection, and mock transfectants (–ve),
were probed with anti-Mvl antiserum incubated overnight without peptide (A) or
incubated with 25 µg of each peptide (B). Proteins were detected by ECL and
size markers of the standard proteins. (Below) The amido black stain of the
two membranes following immune detection.
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