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Fig. 3. (A) Changes of temperature have a greater effect on the neuronal conduction velocity of axons in the leg nerve of C. maenas than in the other three crustacean species (Kruskal–Wallis, ²=13.36, P<0.01). (B) Conduction velocity at 2°C is greater in C. maenas (Kruskal–Wallis, ²=19.55, P<0.0001) than in the other species. Slopes and intercepts were calculated by performing a linear regression on the temperature vs conduction velocity for each animal of each species. The N values are the number of animals per species used. Each boxplot shows the median value, the upper and lower quartiles and the minimum and maximum values. Outliers, which are either 1.5–3 times or >3 times the interquartile range from the quartiles, are denoted by a circle or an asterisk, respectively. The conduction velocity and thermal dependence of conduction velocity of sensory neurones in two G. antarcticus (G. a. sensory) were similar to values for the whole nerve. We acclimated two additional G. antarcticus to 4°C for 7 days before measuring leg nerve conduction velocity (G. a. acclim.). These values (solid circles) lie within the range for animals acclimated to 0–1°C (A,B). The light grey shading groups together all the values from G. antarcticus.

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