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Figure 5


Fig. 5. Effects of Na+-free saline on basolateral membrane potential and intracellular pH in 10–6 mol l–1 5-HT stimulated Malpighian tubules. Representative simultaneous recordings of basolateral membrane potential (A) and intracellular pH (B). Downward-pointing arrow indicates impalement of the cell and upward-pointing arrow the removal of the electrode from the cell. Tubules were exposed to Na+-free saline for the period indicated by the horizontal bar. (C) pHi (mean + s.e.m.) before and 2 min after exposure to Na+-free saline (N=3). The asterisk indicates a significant difference (P<0.05, paired Student's t-test).





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