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Figure 4


Fig. 4. Effects of amiloride 10–5 mol l–1 on fluid secretion rate, basolateral membrane potential and intracellular pH in 10–6 mol l–1 5-HT stimulated Malpighian tubules. (A) Mean fluid secretion rates (± s.e.m., N=7) are shown for tubules exposed to 10–5 mol l–1 amiloride (filled symbols) or to the vehicle alone (open symbols). The arrow indicates the addition of amiloride. (B) Representative recording of basolateral membrane potential. Downward-pointing arrow indicates impalement of the cell and upward-pointing arrow the removal of the microelectrode from the cell. Tubules were exposed to 10–5 mol l–1 amiloride for the period indicated by the horizontal bar. (C) pHi was measured before (control) and ~2 min after addition of amiloride (C, N=4 tubules).





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