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Figure 3


Fig. 3. Effects of bumetanide on basolateral membrane potential, transepithelial potential (TEP), intracellular pH and lumen pH in 10–6 mol l–1 5-HT stimulated Malpighian tubules. Representative simultaneous recordings of TEP (A), lumen pH (B), basolateral membrane potential (Vbl) (D) and intracellular pH (E). Downward-pointing arrows indicate impalement of the cell or lumen and upward-pointing arrows indicate the removal of the microelectrode from the cell or lumen. Tubules were exposed to 10–5 mol l–1 bumetanide for the period indicated by the horizontal bar. Values are means ± s.e.m. for lumen pH (C, N=7) and intracellular pH (F, N=4) before and after bumetanide treatment. The asterisk indicates a significant difference (P<0.05, Student's t-test) between control and experimental values (means + s.e.m.).





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