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Fig. 5. In situ hybridizations of cryostat sections of the compound eye of
H. erato using UVRh, BlueRh and LWRh
digoxigenin-labeled antisense riboprobes. (A) UVRh and (B)
BlueRh opsin mRNAs, respectively, detected using an alkaline
phosphatase-conjugated anti-digoxigenin antibody. The insets show the
magnification of the boxed region. Since the sections are consecutive, the
position of the same ommatidium in A can be identified in B, as indicated by
aligning the black circles. The butterflies express these two opsin mRNAs in
three different ways in different ommatidia: S-S, S-M and M-M in the R1 and R2
photoreceptor cells. (C) Expression pattern of the LWRh opsin mRNA
transcript. In every ommatidium in the main retina, six photoreceptor cells
express the L opsin mRNA. (D) Close up view of the L opsin mRNA expression
pattern in the R3-8 photoreceptor cells. Sections are at around 160 µm from
the cornea. Scale bars, 50 µm (A–C); 20 µm (D).
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