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Fig. 8. Localisation and quantitative distribution of epithelial calcium channel
(ECaC) and Na+/K+-ATPase protein in suspensions of
rainbow trout (Oncorhynchus mykiss) gill epithelial cells using
(A–E) immunocytochemistry and (F) flow cytometry. In A–E, the cell
nuclei appear blue, ECaC appears green and Na+/K+-ATPase
appears red; co-localization of ECaC with Na+/K+-ATPase
appears yellow. (A,B) Representative images of crude cell suspension prior to
separation of different cell types: (1) ECaC-positive cells, (2)
Na+/K+-ATPase-positive cells and (3) cells co-expressing
ECaC and Na+/K+-ATPase. (C) A representative image from
a cell suspension enriched with pavement cells (PVCs). (D,E) Representative
images from purified cell suspensions enriched with peanut lectin
agglutinin-negative (PNA–) mitochondria rich (MR) cells. (F)
The distribution of single- and double-labelled events (cells) as determined
by flow cytometry in cell suspensions enriched with PVCs, filled bars;
N=4) or PNA– MRCs (unfilled bars; N=4).
Data are shown as means ± 1 s.e.m.; *significant difference between the
two cell populations (rank-sum test, P<0.05).
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