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Fig. 5. (A) Representative and (B) mean tissue distributions of epithelial calcium
channel (ECaC) in rainbow trout (Oncorhynchus mykiss) as determined
through RT–PCR and real-time RT–PCR, respectively. ECaC-QPCR1 and
ECaC-QPCR2 primers were used for PCR detection of ECaC, and ß-actin-FWD
and ß-actin-REV primers were used for the detection of ß-actin (see
Table 1 for primer sequences).
For each tissue, a no template control (NTC) sample was tested (data not shown
for real-time PCR results). For real-time PCR, the results are presented as
the expression of ECaC relative to ß-actin and standardised to ECaC
expression in the gill (N=4).
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