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Fig. 8. A peptide directed against the interaction domain between TRPC2 and
IP3R3 blocks the chemosignal-activated currents in S.
odoratus vomeronasal sensory neurons (VSNs) when included in the patch
pipette in the whole-cell configuration. (A) Amino acid sequence of the
synthesized peptide (derived from mTRPC2, amino acids 905–934)
(Tang et al., 2001). (B)
Histogram plot of the chemosignal-activated current over 10 min when 10
µmol l–1 peptide is included in the recording pipette. The
normal chemosignal-activated current over time is denoted by a broken line
(see Fig. 4). Current obtained
for each cell at varying time points (tn) was normalized
to its first exposure to chemosignal (t0) Asterisks denote
significant differences between control and peptide, two-way repeated measures
ANOVA, followed by SNK pairwise multiple comparison between treatment and
time, P
0.05). (C) Representative example of peptide treatment.
While in the whole-cell configuration, the VSN was presented with a
chemosignal, and a baseline (immediately after the whole-cell configuration
was obtained) recording was made (top). The same chemosignal-activated current
is shown 10 min after peptide infusion (bottom). Broken line denotes baseline
current.
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