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Figure 5


Fig. 5. Dialysis of the second messengers cAMP, IP3, OAG, arachidonic acid (AA), and SAG in isolated S. odoratus vomeronasal sensory neurons (VSNs). Analogues were made as described (see Materials and methods) and back-filled in the recording pipette to allow diffusion upon attaining the whole-cell configuration. VSNs were voltage-clamped at Vh=–60 mV. (A) A representative example of a VSN that had not been dialyzed with a second messenger analogue in order to illustrate stability of the patch. (B) 0.5 or 1 µmol l–1 cyclic adenosine monophosphate (cAMP) (N=27). (C) 240 µmol l–1 inositol 1,4,5-trisphosphate (IP3) (N=20). (D) 125 µmol l–1 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol (DAG), applied to either the bath surrounding the VSN or within the recording pipette (N=15). Trace shown is an example of pipette perfusion. (E) 10 µmol l–1 AA, a polyunsaturated fatty acid (PUFA) derivative of DAG (N=11). (F) 10 mmol l–1 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG), a natural analogue of DAG (N=6).





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