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Fig. 4. Ruthenium Red (RR), an antagonist of the IP3R, fails to block
chemosignal-activated currents in S. odoratus vomeronasal sensory
neurons (VSNs) while an inhibitor of phospholipase C (U73122) does block
chemosignal-activated currents. (A) A representative example of the response
of a female VSN to catfish extract at 0 min (top trace) and 6 min later
(bottom trace) without pharmacological perturbation. The chemosignal
presentation is shown as black bar above the response. This particular cell
was used primarily for reversal potential analysis, hence the VSN had been
exposed to the chemical signal twelve times within the time span described.
(B) Histogram plot of the normalized current magnitude (normalized to first
exposure to chemosignal; t0) over time. Cells were sampled
at varying time points (tn), such that not every cell was
sampled for each of the time points. The amplitude of the
chemosignal-activated current was not altered over 12 min (one-way repeated
measures ANOVA, P
0.05). (C) A representative example of the
response of a female VSN to male urine at 0 min (top trace) and 6 min (bottom
trace) following bath application of 1 mmol 1–1 RR. (D)
Histogram plot of the normalized current magnitude (normalized to
t0) over time. The chemosignal-activated current is not
altered over 10 min (not significantly different by treatment or time, two-way
repeated measures ANOVA, P0.05) in response to RR treatment. The
normal chemosignal response over time is denoted by the broken line. (E) A
representative example of the response of a female VSN to catfish extract at 0
min (top trace) and 8 min (bottom trace) following bath application of 50
µmol l–1 U73122. (F) As in D but for U73122 treatment.
Asterisks denote significant difference between treatments, two-way repeated
measures ANOVA, followed by SNK pairwise multiple comparison between treatment
and time, P0.05). N values are shown beside bars.
(A,C,E) Broken line denotes baseline current.
