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Fig. 3. Reduced Dms transcript levels in the CNS of dimm mutant, but not crc mutant, hatchling larvae. (A) In situ hybridization with a Dms antisense probe in dimm^KG02598/+ CNS. (B–D) Intensity of Dms in situ hybridization for the MP2, SE and SP cells in (B) dimm^KG02598/Rev4 (N=9) vs dimm^KG02598/+ (N=11), (C) Rev8/Rev8 (N=12) vs Rev8/+ (N=13), and (D) crc¹/crc¹ (N=5) vs crc¹/+ (N=11) larvae. Paired genotypes were processed for in situ hybridization in parallel within each experiment (e.g. Rev8/Rev8 vs Rev8/+) but not between experiments (e.g. B vs D), and the baseline in situ hybridization intensities between experiments cannot be directly compared. *P<0.05; ***P<0.001; one-way ANOVA. Scale bars: 25 µm (A); 2.5 µm (B–D).

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