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Fig. 5. AIY neurons function to extend food-seeking periods. Trajectory on mediocre
food, E. coli DA837, of (A) a ttx-3 mutant and (B) an animal
whose AIY neurons have been killed. Compare to wild-type in
Fig. 4C. ttx-3 mutant
trajectories did not span the whole lawn; and there were far fewer long
straight roaming events. Trajectories of AIY worms also had fewer straight
long movements than wild-type controls. (C,D) Movement duration distribution
of (C) wild type, ttx-3, osm-6, osm-6;ttx-3 and (D) AIY-ablated
animals, all tested on E. coli DA837 food. N=10 for WT, 10
for ttx-3, 6 for osm-6, 6 for osm-6;ttx-3, 10 for
AIY ablations and 8 for ttx-3p::GFP controls. (E) ttx-3 was
defective in the food preference behavior if bacterial foods were located at a
small distance from each other. By 3 h, all ttx-3 worms found food,
but there was no preference in the harder arrangement. In contrast to
ttx-3, osm-6 animals took longer to discriminate between good and bad
food, but they finally managed to make the right choice even if foods were
located at a distance. Values are means ± s.e.m. *Different
from the wild type (P<0.01); 
different from
ttx-3 (P<0.01; Student's t-test). (F) Biased
food preference for E. coli HB101 over B. megaterium of
mutants and animals with laser-ablated neurons. The fraction of animals that
reached the central colony of good food, E. coli HB101, was
determined. ttx-3 mutants and AIY-ablated animals performed worse
than controls. In laser ablation experiments, worms were counted after 20 h.
For tests on mutants, the number of assays is 18 for WT, 15-17 for
ttx-3 alleles and 6-15 for various mutants tested. For laser
ablations, number of worms found in the center and the total number of worms
tested is indicated next to the bars. Values are means ± s.e.m.
*Different from the wild type (P<0.01; Student's
t-test); Different from the ablation control
(P<0.01; 
2 test of independence).
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