|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Effect of dietary experience on food seeking behavior. (A,B) Effect of
experience on food choice. (A) Wild-type L1 larvae were kept for 2 h in one of
the four conditions, and then each group was tested in the `harder' choice
assay with three food combinations. The only two cases where the consistent
time course of the food choice was observed were groups conditioned on
Comamonas and tested on pairs of E. coli DA837 vs E.
coli HB101, and E. coli DA837 vs Comamonas; in both
cases worms chose better food. The effect of experience seems mild, but note
that without conditioning, no preference develops with these pairs in a harder
arrangement (Fig. 1A). Values
are means ± s.e.m. (N=5). *Different from worms
conditioned on E. coli DA837 and empty plate (P<0.02;
Student's t-test). (B) Wild-type larvae were exposed to one of the
listed conditions for 2 h and then tested in a biased choice assay with a
circle of DA837 surrounding a central colony of HB101. Worms that have
experienced the high quality food, Comamonas and E. coli
HB101, show the strongest food choice. Values are means ± s.d.
(N=20; 10 plates with two circle assays on each). At all time points
groups conditioned on food are different from those conditioned on the control
empty plate (P<0.01; Student's t-test). (C) Effect of
food experience on leaving behavior. 50-80 naive eat-2 L1 larvae were
conditioned in one of the five indicated conditions for 3 h, washed and
transferred to another plate for a leaving assay. The time when the first worm
entered the colony is time point 0. The x-axis shows the time
intervals within which leaving probability was determined: 0-30 min, 30 min-1
h, 1-2 h, and in 1-h increments thereafter. After exposure to high quality
food, Comamonas or HB101, leaving behavior was increased as compared
to conditioning on the same food, worse food, or without food (empty plate).
Values are means ± s.e.m. (N=6). *Different from
worms conditioned in any of the other conditions (P<0.05;
Student's t-test).
|
