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Fig. 6. Immunostaining with antisera against amino acids and escapin in ink gland
of Aplysia californica. Tissue is 0.5 µm thick plastic sections.
A, B and C are from three different ink glands, each a set of four sections
from the same region of tissue labeled with different antisera, i.e. antiserum
against either escapin, alanine, taurine, arginine, cysteine or aspartate.
`Preimmune' is the control, being labeled with preimmune serum. Vesicles
labeled `a' are strongly immunopositive for many amino acids but not escapin;
vesicles labeled `e' are intensely immunopositive for escapin but not for
amino acids; vesicles labeled `c' are clear and not strongly immunopositive
either for amino acids or escapin.
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