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Fig. 5. Changes in the accumulated mRNA levels of carp MYH isoforms during
ontogeny. Total RNAs (20 µg) were electrophoresed in a 1% agarose gel and
transferred on to the nylon membranes, which were then hybridized with
32P-labeled probe. Lanes contain total RNAs from the whole embryos
at 30 h, 42 h, 61 h, and 77 h.p.f., from the whole larvae at hatching (96 h),
from the muscle of juveniles aged 1 and 7 months post-hatching and from the
fast and slow skeletal muscles of thermally acclimated adult fish. An ethidium
bromide-stained gel shows 28S and 18S rRNA. Consensus indicates the membrane
hybridized with the probe, which is supposed to react with all skeletal MYH
mRNA of carp. Each lane contains 5 µg of total RNA.