|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Inhibitor analysis to determine the cost of protein synthesis in embryos
and larvae of L. pictus. Protein synthesis (PS) and metabolic rates
(O2) in the presence and absence of emetine (see Materials and
methods for details) are shown for each replicate measurement for each
developmental stage studied (blastula, gastrula and larva). Total height of
each bar represents metabolic rate or protein synthesis rate when no inhibitor
was present. The height of the solid component represents the rate in the
presence of emetine. Error bars are calculated as s.e.m. of the slope for each
linear regression analysis (error of the estimate of protein synthesis
calculated as in Fig. 1C; error
for metabolic rate as in Fig.
1F). Costs of protein synthesis (all means ± s.e.m.) were:
blastula, 7.76±2.55 J mg-1 protein (N=3); gastrula,
7.75±0.90 J mg-1 protein (N=3); larva,
10.34±0.57 J mg-1 protein (N=2). The costs were not
significantly different for all stages of development analyzed (ANOVA;
P=0.60, N=8). The average cost of protein
synthesis=8.40±0.99 J mg-1 protein.
|
