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Fig. 5. Innervation of neuroepithelial cells in developing gill filaments of 7 and
9 d.p.f. larvae. (A) Serotonin (5-HT)-immunoreactivity (IR) of an isolated
gill from a larva at 7 d.p.f. At this stage, filaments (f) were longer and
primordium of respiratory lamellae (lam) were observed. A single 5-HT-IR
neuroepithelial cell (NEC, green) resided within the developing filament.
Scale bar, 10 µm. (B) Image in A shown with zn-12-IR indicates that the NEC
was associated with zn-12-IR nerve fibres (arrowheads) that arose from a nerve
bundle (nb) of the gill arch (ga). A zn-12-IR neuron (n) is also visible. (C)
5-HT-IR of an isolated gill from a larva at 9 d.p.f. A 5-HT-IR NEC (green) of
the filament (f) and a 5-HT-IR NEC of the gill arch (ga) were present. Scale
bar, 10 µm. (D) Image in C shown with zn-12-IR reveals that both of these
cells were associated with zn-12-IR (red) nerve fibres (arrowheads) arising
from a nerve bundle (nb) of the gill arch (ga).
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