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Fig. 7. Example of a typical histogram showing the movement of larval fish in
quiet. For a data set consisting of 56 trials, the first 2.32 s of each trial
were frame subtracted and the number of pixels above the noise threshold was
determined for a moving window of three frames. The frequency of occurrence of
the number of pixels above the noise threshold is plotted as a histogram, and
a single exponential equation was fit to the histogram (dotted line; goodness
of fit 
=0.1012). In this case, a positive response would be registered
if the cumulative number of pixels above the noise threshold when the tone was
on (frames 60 to 62) was >91 (P<0.0001; arrowhead).
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