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Fig. 6. Examples of video frame subtraction. (A,B) Show the subtraction of two different consecutive frames for the fish in quiet. In A, no movement occurs between frames and the resulting difference image (column 3) contains only noise. In B, two fish move resulting in two darker patches in the difference image. C shows several fish moving in the presence of the stimulus. Note that in areas where no movement takes place, the noise is small and consistent in the difference images. Fish are 4.5 mm (16 dpf).





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