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Fig. 5. Progression of gene expression of the five opsin types (RH1, M/LWS, SWS1,
RH2 and SWS2) in embryonic zebrafish retina sampled at 40, 45, 50, 55, 60 and
72 hpf. Embryos sampled at each hpf were examined for staging opsin expression
by whole-mount ISH with CDR probes of the five opsin genes (total number of
embryos examined, N, are indicated in each panel). The number of
embryos (vertical axis) showing certain expression stages (horizontal axis)
was counted at each hpf for each gene. One eye was examined per embryo. Stage
0 indicates that no expression was detected in an observed retina. For RH1,
subsequent stages are defined as follows
(Raymond et al., 1995;
Hamaoka et al., 2002): stage
1–2, expression is detected in a patch of ventronasal retina; stage 3,
expression is detected across the choroid fissure from the primary ventronasal
patch; stage 4, hybridization signal increases in the ventrotemporal patch and
scatters outside the ventral region; stage 5–6, ventrotemporal and
ventronasal patches are at equivalent density and fuse across the fissure to
form a dumbbell shape, and density of the signal outside the ventral region
further increases. For cone opsins (M/LWS, SWS1, RH2 and SWS2), refer to
Fig. 1 legend for definitions
of stages.
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