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Fig. 2. Aeropyle density and cross-sectional area of eggs of Manduca sexta. (A) Light microscope view (400x, immersed in oil) of the surface of a chorion removed from the egg and washed in saline. Polygons are the imprints of the follicular cells that deposited the chorion (Orfanidou et al., 1992). White arrows point to two aeropyles; about 13 aeropyles are visible in this field. (B) SEM view of the outer surface of a chorion. Polygons are not visible, but dimples (see A) are. A single aeropyle is in the upper left quadrant. (C) Close up view of a single aeropyle. (D) Histogram of aeroyple cross-sectional areas. Data represent 10 aeropyles from each of five eggs (N=50). Mean cross-sectional area (±S.E.M.) is 0.775 ± 0.054 µm2.





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