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Fig. 5. Quantitative evaluation of isolated Calliphora rhabdoms labelled
for Rh3, Rh5 and Rh6. Isolated Calliphora rhabdoms were double
labelled with antibodies specific for Rh5 and Rh6 (A,B) or with antibodies
against Rh3 and Oregon Green-coupled wheat germ agglutinin (C,D). (A) An
overlay of differential interference contrast and indirect immunofluorescence
images of two rhabdoms, one labelled with the anti-Rh5 antibody (red) and the
other one labelled with the anti-Rh6 antibody (green). (B) The percentage of
rhabdoms containing Rh5 or Rh6 was determined by counting the number of
rhabdoms stained with either antibody. (C) A few rhabdoms viewed with
differential interference contrast optics, one of which is labelled with
anti-Rh3 (red). (D) The percentage of rhabdoms stained with anti-Rh3 was
determined relative to the total number of rhabdoms stained with wheat germ
agglutinin. For the quantitative evaluation, four independent preparations
from both male and female flies were analysed. Values are means ±
S.D. Scale bar, 40 µm.
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