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Fig. 3. Specific immunolabelling of Calliphora rhodopsins Rh3, Rh5 and Rh6. Longitudinal cryosections through the compound eye of Calliphora were probed with antibodies directed against Calliphora Rh3 (A,B), Calliphora Rh5 (C,D) and Drosophila Rh6 (E,F) in the absence or presence of peptides as indicated. The peptides used to block the reaction with Calliphora Rh3 (25 µg ml–1) and Rh5 (15 µg ml–1) were the same as used to generate the polyclonal antibodies. Reaction of the monoclonal anti-Drosophila Rh6 antibody was blocked with a peptide of the C-terminal region of Calliphora Rh6 rhodopsin (CGKDDTASDSRTQATA, 25 µg ml–1). Indirect immunofluorescence of antibodies is shown in green. Red fluorescence: labelling of actin filaments with rhodamine-coupled phalloidin. Scale bar, 80 µm.





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